Long fragments, modifications and disease-specific panels
On the one hand, the latest methods such as nanopore or SMR technology (single-molecule real-time sequencing) can be used to read ever longer DNA fragments of up to several tens of thousands of base pairs at a time.
On the other hand, more and more specialized systems are being developed with which epigenetic modifications such as methylations or binding sequences of transcription factors can be analyzed. In addition, so-called panels can be used to specifically detect certain disease markers in the blood. This allows, for example, a return of cancer or mutations in tumor cells to be detected at an early stage. This means that targeted countermeasures can be taken more quickly.
Unique expertise in integrated quality inspection
HSE•AG’s experience in qualifying the quality of nucleic acid samples is unique. It ensures that no contaminated or overly diluted samples pass through the sequencing process and then produce unreliable results.
“With our OEM component eviDense UV, for example, the concentration and purity determination of NGS samples can be integrated directly into a liquid handler,” says Geipel.
The module, which only requires one microtiter plate space, uses four wavelengths in the UV range for the analysis of protein, RNA, salt or organic impurities and for precise concentration determination. Thanks to a sophisticated process, the analysis does not consume any valuable sample material.
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