High-precision automated PCR setup

The QIAgility is a compact benchtop workstation used to automatically set up PCR assays with exceptionally high-precision. Since all manual pipetting steps are eliminated, QIAgility can ensure both reproducibility and increased productivity. It features a single channel pipetting head, delivering fast and safe transfer of liquids, and features dynamic liquid-level sensing with a minimum detection volume of 10 µl.

Pure pipetting accuracy

The reproducible and highly accurate PCR setup mainly relies on the exceptionally high quality of the dispensations, which range between 1 µl and 200 µl. This is enabled by the QIAgility Setup Manager software and the precision of the dispensed volumes has been verified by repeated tests in various settings. The high sensitivity of the system is ensured by its ability to sense the meniscus during pipetting and retracting the tip during dispensation, thereby increasing the accuracy and minimizing any carry-over. If the liquids are viscous, the pipetting speed will be adapted accordingly. Tests with serial dilutions have proven that the linearity of system is exceptional, with a regression coefficient of >0.999. Moreover, the software provides step-by-step guidance and automatically calculates all mixes, so that no pipetting steps need to be programmed. Any contamination of the samples is prevented by an optional UV light and the HEPA filter system ensures positive pressure of clean air within the instrument. In addition, all used tips are ejected externally, so that no waste is accumulated on the worktable.

Flexibility and applications

The PCR assay setup performed by QIAgility can easily be integrated into various workflows. Almost all tube and plate formats are supported, such as 96-well and 384-well plates, but also Rotor-Discs for the Rotor-Gene® Q real-time PCR instrument. For added convenience, a laptop computer with intuitive software is provided. The user can program various pipetting applications, such as normalization of DNA and RNA concentration, transfer of liquid samples between different tube formats, serial dilutions with variable dilution ratios, restriction digest setups, sample pooling and selective pipetting from archived sample banks.