Dedicated, fearless, tenacious, passionate – these are just a few attributes to describe my dear colleague and good friend Mirjana.
Efficient single-cell isolation
The QIAscout is a device based on magnetic technology and is used to efficiently isolate and recover single cells. The isolation can be carried out within minutes and the method ensures minimal manipulation of the recovered cells. After the isolation, the isolated cells can be used for direct analysis or clonal expansion. The procedure only requires a cell sample, QIAscout and an inverted microscope. The device comes with an array coated with magnetic nanoparticles and packed with 12,000 microrafts, which serve as releasable culture sites for individual cells or clones. The system also includes a release device, a magnetic wand and a magnetic collection plate.
Simple and straight-forward
The workflow is exceptionally easy. Initially, the cells have to be seeded and cultivated on the QIAscout array. The release device with its release needle is then placed on the microscope objective, while the array is placed on the microscope stage. As soon as the cells of interest have been identified, the corresponding microrafts are pierced, dislodged and transferred to vessels, e.g. reaction tubes or cell culture dishes, using a magnetic wand. Since the cell viability and purity is maintained, as proven by several studies, the collected cells can be used for several applications.
The QIAscout is intended for molecular biology applications, including single-cell sequencing, whole genome and transcriptome amplification and single-cell miRNA analysis, as well as generation of clonal subpopulations. QIAscout can be used for a variety of eukaryotic cell types and the selection of cells can be based on morphology, fluorescence or other parameters, making it even easier to integrate the method into existing workflows. Moreover, the cells are viable for several days after the selection, giving the user more flexibility in designing the workflow.