Precise and robust real-time PCR

The real-time PCR-cycler Rotor-Gene Q is designed to be used for real-time and end-point thermal cycling using polymerase chain reaction (PCR) and high-resolution melting analysis (HMR) for various molecular biology applications. It has a unique design with a rotor that spins the samples, which minimizes the optical and thermal variation. The instrument’s stability in combination with its fast ramping rates and short equilibrium times renders Rotor-Gene Q highly suitable for fast, sensitive and accurate quantitative analyses.

User-friendly design and software

With its small, light and robust design there is hardly any need for maintenance. There is a lifetime guarantee for the LEDs and no need for optical calibration or cleaning of sample blocks. Also, the rotary design prevents any condensation or bubbles forming in the reactions. Rotor-Gene Q can be used with either tubes or Rotor-Discs for faster handling of high-throughput workflows. In addition, it features intuitive software, such as the Q-Rex Software, which supports all analysis procedures from basic to advanced algorithms, and its extension software Rotor-Gene ScreenClust HRM Software, which enables HRM analysis for genotyping, mutation screening, pathogen typing and quantitative methylation analysis. Furthermore, the software REST software 2009 takes the different PCR efficiencies of the gene of interest and reference genes into account and provides analysis of e.g. up- and downregulation for gene expression studies.

Widest optical range — even more applications

Rotor-Gene Q has an unrivaled optical range with up to 6 channels spanning from UV to infrared wavelengths and can therefore be used with a number of different fluorophores. The software also allows addition of new wavelength combinations so that it will be compatible with new dyes to be used in future applications. Today, the instrument can be used for applications such as gene expression analysis, genotyping, pathogen detection, mutation analysis, DNA methylation analysis and miRNA research.

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