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Medium- to high-throughput sample disruption
The TissueLyser II is designed for medium- to high- throughput disruption of human, animal and plant tissues as well as bacteria and yeast samples. The process is based on bead mill technology, with high-speed shaking in plastic tubes with stainless steel, tungsten, carbide or glass beads, which are 3 mm, 5 mm or 7 mm in diameter. Bead Dispensers are provided to ensure that the correct amount of beads is dispensed into the samples. The tissues can also be processed with grinding jar sets made of Teflon or steel. Up to 48 or 192 samples can be processed simultaneously, and the provided adapter sets ensure the disruption optimal for the chosen quantity.
The release of nucleic acids from the sample material relies on proper disruption followed by homogenization of the starting material. TissueLyser II performs both, disrupts and homogenizes the material, making sure that high-molecular-weight cellular proteins and carbohydrates are also sheared, as opposed to a mortar and a pestle, which only disrupt the material. Thorough shearing of these molecules is a prerequisite for efficient purification of nucleic acids in downstream applications. The sample homogenization is achieved by running the TissueLyser II at variable speeds from 3 to 30 Hz (180–1800 oscillations/minute) and the run times range between 10 seconds and 99 minutes.
The TissueLyser II is suitable for convenient and secure disruption and homogenization of a wide range of biological starting materials. Even samples that are normally difficult to lyse, such as heart and brain tissues can be successfully processed using the TissueLyser II, ensuring that the released DNA, RNA and proteins remain intact. The high performance of the instrument renders it a useful tool in any large-scale molecular biology applications that require rapid homogenization of samples, as wells as high sample quality.