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Low- to medium-throughput sample disruption
The TissueLyser LT is designed for low-to medium throughput disruption of human, animal and plant tissues as well as bacteria and yeast samples. The process is based on bead mill technology, with high-speed shaking in plastic tubes with stainless steel or glass beads which are 5 mm or 7 mm in diameter. Bead Dispensers are provided to ensure that the correct amount of beads is dispensed into the samples. Up to 12 samples can be processed simultaneously and a cooled adapter prevents degradation of the biomolecules.
The release of nucleic acids from the sample material relies on proper disruption followed by homogenization of the starting material. TissueLyser LT performs both, disrupts and homogenizes the material, making sure that carbohydrates also are sheared, as opposed to a mortar and a pestle, which only disrupt the material. Thorough shearing of carbohydrates is a prerequisite for efficient purification of nucleic acids in downstream applications. The sample homogenization is achieved by running the TissueLyser LT at variable speeds from 15 to 50 Hz (900–3000 oscillations/minute) and the run times ranges between 1 second and 1 hour 59 minutes.
Quality and versatility
The TissueLyser LT is suitable for disruption and homogenization of a wide range of biological starting materials. Even samples that are normally difficult to lyse, such as heart and brain tissues can be successfully processed using the TissueLyser LT, ensuring that the released DNA, RNA and proteins remain intact. The high performance of the instrument in combination with its compact design, the coolable adapter and its compatibility with all laboratory workflows renders it a useful tool for any small-scale molecular biology applications that require rapid homogenization of samples, as wells as high sample quality.